Appl Microbiol Biotechnol (2020): “Engineering novel S-glycosidase activity into extremo-adapted β-glucosidase by rational design”
This paper presents Nourah’s work on how strategic site directed mutagenesis has been successfully applied to shift substrate preference in glycosyl hydrolases. The mutant enzyme is now able to work with thio-glycosides which are found in several plants and have shown both antimicrobial and anticancer activity. In fact the identified positions could be used also in a second similar enzyme which displayed similar behaviour. Nick helped Nourah on the kinetic evaluation which were quite challenging.You can find the paper here.