Chemical Communication (2022): “Novel triple mutant of an extremophilic glycosyl hydrolase enables the rapid synthesis of thioglycosides”

In order to expand the toolbox of enzymes available for thioglycoside synthesis, we describe here the first example of an extremophilic glycosyl hydrolase from Halothermothrix orenii (HorGH1) engineered towards thioglycosynthase activity with a novel combination of mutations. Using the triple mutant, HorGH1 M299R/E166A/E354G, a range of thioglycosides from glycosyl fluoride donors and aromatic thiols could be synthesised with […]

Food Chemistry (2020): “Performance of the extremophilic enzyme BglA in the hydrolysis of two aroma glucosides in a range of model and real wines and grape juices”

Very well done Lidia on successfully completing this challenging project! A very good results from the time spent in Australia, and for sure finding new ways to improve aromaticity in wines is ideal at the time of this quarantine…Here we have exploited an extremophilic glycosyl hydrolases which is showing exceptional tolerance to high concentrations of […]

Appl Microbiol Biotechnol (2020): “Engineering novel S-glycosidase activity into extremo-adapted β-glucosidase by rational design”

This paper presents Nourah’s work on how strategic site directed mutagenesis has been successfully applied to shift substrate preference in glycosyl hydrolases. The mutant enzyme is now able to work with thio-glycosides which are found in several plants and have shown both antimicrobial and anticancer activity. In fact the identified positions could be used also […]