Chemistry: a European Journal (2020): “Carbene-induced rescue of catalytic activity in deactivated Nitrite Reductase mutant”
Finally we have succeeded in getting this final work of Matteo over the line! This is the second one in the series of the carbene role in binding copper in protein clefts. Here we have shown how a catalytically active enzyme NiR can be partially rescued by replacing a coordinating histidine with the carbene exogenous ligand. In fact, this ligands […]
Frontiers Bioeng. Biotechnol. (2020): “An (R)-Selective Transaminase from Thermomyces stellatus: Stabilizing the Tetrameric Form”
Chris expanded our transaminase toolbox by cloning a new (R) selective enzyme which has the advantage of expressing in very high quantities and it’s very stable. Together with out collaborator Louise Gourlay in Milan, we have also successfully crystallised the structure and provided very useful insight into the quaternary structure of such enzyme. The tetrameter could be further stabilised by introducing strategic cysteine residues to enable the formation of intra-subunit […]
Food Chemistry (2020): “Performance of the extremophilic enzyme BglA in the hydrolysis of two aroma glucosides in a range of model and real wines and grape juices”
Very well done Lidia on successfully completing this challenging project! A very good results from the time spent in Australia, and for sure finding new ways to improve aromaticity in wines is ideal at the time of this quarantine…Here we have exploited an extremophilic glycosyl hydrolases which is showing exceptional tolerance to high concentrations of […]
Appl Microbiol Biotechnol (2020): “Engineering novel S-glycosidase activity into extremo-adapted β-glucosidase by rational design”
This paper presents Nourah’s work on how strategic site directed mutagenesis has been successfully applied to shift substrate preference in glycosyl hydrolases. The mutant enzyme is now able to work with thio-glycosides which are found in several plants and have shown both antimicrobial and anticancer activity. In fact the identified positions could be used also […]
Green Chemistry (2019): “Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process”
To increase the uptake of biocatalytic processes by industry, it is essential to demonstrate the reliability of enzyme-based methodologies directly applied to the production of high value products. Here, an ultra-efficient, and sustainable enzymatic platform for the multi-gram synthesis of melatonin (and analogues) was developed. The fully-automated process integrates biocatalysis and flow chemistry, maximizes the […]
Nature Catalysis (2018): Self-sustaining closed-loop multienzyme-mediated conversion of amines into alcohols in continuous reactions
Massive congratulation to Martina for this achievement! The paper summarises an outstanding amount of work with very clever applications. Find the paper here Or you can access a read only version here Related Posts