ChemBioChem (2022): “Fusion of Formate Dehydrogenase and Alanine Dehydrogenase as an Amino Donor Regenerating System Coupled to Transaminases”

Another paper from Valentina with the contribution of our master student Sopha and Ana. Abstract: A fusion protein combining alanine dehydrogenase and formate dehydrogenase has been successfully produced as a bifunctional enzyme. Two different genetic designs by swapping the position of both protein domains were developed, but one construct showed up to 2.5-fold higher enzymatic activity than […]

Catalysis Science & Technology (2022): “A novel thymidine phosphorylase to synthesize (halogenated) anticancer and antiviral nucleoside drugs in continuous flow”

Great work from Ana, Calvin and David! Abstract: Four pharmaceutically relevant nucleoside analogues (5-fluoro-2’-deoxyuridine, 5-chloro-2’-deoxyuridine, 5-bromo-2’-deoxyuridine, and 5-iodo-2’-deoxyuridine) have been synthesized by using a novel thymidine phosphorylase from H. elongata (HeTP). Following enzyme immobilization on microbeads, the biocatalyst was implemented as a packed-bed reactor for the continuous production of halogenated nucleosides, achieving up to 90% […]

ChemBioChem (2022): “Stereo-divergent enzyme cascades to convert racemic 4-phenyl-2-butanol into either (S)- or (R)- corresponding chiral amine”

ChemBioChem (2022): “Stereo-divergent enzyme cascades to convert racemic 4-phenyl-2-butanol into either (S)- or (R)- corresponding chiral amine” The synthesis of enantiopure chiral amines from racemic alcohols is a key transformation in the chemical industry, e. g., in the production of active pharmaceutical ingredients (APIs). However, this reaction remains challenging. In this work, we propose a one-pot […]

Scientific Reports (2022): “High-throughput screening methods for enzyme-mediated alcohol oxidation”

Scientific Reports (2022): “High-throughput screening methods for enzyme-mediated alcohol oxidation” Alcohol oxidation for the generation of carbonyl groups, is an essential reaction for the preparation of fine chemicals. Although a number of chemical procedures have been reported, biocatalysis is a promising alternative for more sustainable and selective processes. To speed up the discovery of novel […]

Chemistry- A European Journal (2021): “GPhos ligand enables the production of chiral N-arylamines in a telescoped transaminase–Buchwald-Hartwig amination cascade in the presence of excess amine donor”

A fine paper from Chris. Here he explored how we can combine transaminases and chemocatalysis, specifically looking for water-compatible Pd catalysts to synthesise arylamines. All the credits goes to him and he shares here the corresponding authorship! Chemistry- A European Journal 2021 in press (doi:10.1002/chem.202103472) Read the full paper here Related Posts

Chem Comm (2021): “Minimalistic peptidic scaffolds harbouring an artificial carbene-containing amino acid modulate reductase activity”

Another collaborative paper with the group of Martin Albrecht here at the DCBP, we really like to combine carbenes and natural molecules such as peptides and check out how the catalysis is affected. Matteo worked on the synthesis and David did his usual magic with molecular dynamics and simulations! A great paper to read! Chemical Communication 2021, 57, 9068 – 9071 […]