Nature Communication (2022): “Spheroplasts preparation boosts the catalytic 

Squalene-hopene cyclases are a highly valuable and attractive class of membrane-bound enzymes as sustainable biotechnological tools to produce aromas and bioactive compounds at industrial scale. However, their application as whole-cell biocatalysts suffer from the outer cell membrane acting as a diffusion barrier for the highly hydrophobic substrate/product, while the use of purified enzymes leads to […]

Chemical Communication (2022): “Novel triple mutant of an extremophilic glycosyl hydrolase enables the rapid synthesis of thioglycosides”

In order to expand the toolbox of enzymes available for thioglycoside synthesis, we describe here the first example of an extremophilic glycosyl hydrolase from Halothermothrix orenii (HorGH1) engineered towards thioglycosynthase activity with a novel combination of mutations. Using the triple mutant, HorGH1 M299R/E166A/E354G, a range of thioglycosides from glycosyl fluoride donors and aromatic thiols could be synthesised with […]

Green Chemistry (2022): “Combined chemoenzymatic strategy for sustainable continuous synthesis of the natural product hordenine”

First paper for Stefania! To improve sustainability, safety and cost-efficiency of synthetic methodologies, biocatalysis can be a helpful ally. In this work, a novel chemoenzymatic stategy ensures the rapid synthesis of hordenine, a valuable phenolic phytochemical under mild working conditions. In a two-step cascade, the immobilized tyrosine decarboxylase from Lactobacillus brevis (LbTDC) is here coupled with the […]

ChemBioChem (2022): “Fusion of Formate Dehydrogenase and Alanine Dehydrogenase as an Amino Donor Regenerating System Coupled to Transaminases”

Another paper from Valentina with the contribution of our master student Sopha and Ana. Abstract: A fusion protein combining alanine dehydrogenase and formate dehydrogenase has been successfully produced as a bifunctional enzyme. Two different genetic designs by swapping the position of both protein domains were developed, but one construct showed up to 2.5-fold higher enzymatic activity than […]

Catalysis Science & Technology (2022): “A novel thymidine phosphorylase to synthesize (halogenated) anticancer and antiviral nucleoside drugs in continuous flow”

Great work from Ana, Calvin and David! Abstract: Four pharmaceutically relevant nucleoside analogues (5-fluoro-2’-deoxyuridine, 5-chloro-2’-deoxyuridine, 5-bromo-2’-deoxyuridine, and 5-iodo-2’-deoxyuridine) have been synthesized by using a novel thymidine phosphorylase from H. elongata (HeTP). Following enzyme immobilization on microbeads, the biocatalyst was implemented as a packed-bed reactor for the continuous production of halogenated nucleosides, achieving up to 90% […]

ChemBioChem (2022): “Stereo-divergent enzyme cascades to convert racemic 4-phenyl-2-butanol into either (S)- or (R)- corresponding chiral amine”

ChemBioChem (2022): “Stereo-divergent enzyme cascades to convert racemic 4-phenyl-2-butanol into either (S)- or (R)- corresponding chiral amine” The synthesis of enantiopure chiral amines from racemic alcohols is a key transformation in the chemical industry, e. g., in the production of active pharmaceutical ingredients (APIs). However, this reaction remains challenging. In this work, we propose a one-pot […]

Scientific Reports (2022): “High-throughput screening methods for enzyme-mediated alcohol oxidation”

Scientific Reports (2022): “High-throughput screening methods for enzyme-mediated alcohol oxidation” Alcohol oxidation for the generation of carbonyl groups, is an essential reaction for the preparation of fine chemicals. Although a number of chemical procedures have been reported, biocatalysis is a promising alternative for more sustainable and selective processes. To speed up the discovery of novel […]