Chemical Science (2019): “Widely applicable background depletion step enables transaminase evolution through solid-phase screening”
This paper describes what appears to be an almost trivial solution to a very well known issue in microbial screening for transaminase activity. Quenching the background with a simple neutralization of the basal pyruvate presence was a stroke of genius! Well done to everyone who contributed! And by the way, we have included also the […]
Frontiers Bioeng. Biotechnol. (2019): “Enhancing PLP-binding capacity of class-III ω-transaminases by single residue substitution”
The importance of PLP stability in transaminases prompted us to investigate less obvious residues which may be involved in anchoring the PLP. This work was a great collaboration with Dr. Lopez-Gallego (who just recently moved back to San Sebastian) and Dr.Dreveny in Nottingham. Very well done to David who carried out this work and got the good news of it being accepted on the […]
Chemistry- A European Journal (2021): “GPhos ligand enables the production of chiral N-arylamines in a telescoped transaminase–Buchwald-Hartwig amination cascade in the presence of excess amine donor”
A fine paper from Chris. Here he explored how we can combine transaminases and chemocatalysis, specifically looking for water-compatible Pd catalysts to synthesise arylamines. All the credits goes to him and he shares here the corresponding authorship! Chemistry- A European Journal 2021 in press (doi:10.1002/chem.202103472) Read the full paper here Related Posts
Green Chemistry (2021): “Biocatalytic access to betazole by one-pot multienzymatic system in continuous flow”
Maria successfully tackled a multi enzyme system which requires a NOX enzyme to recycle the NADH cofactor. Not a small battle when you require to maximise the air/oxygen content in a sealed system like a flow reactor. Very proud of this work! Abstract: “As an alternative to classical synthetic approaches for the production of betazole drug, a […]
ACS Sustainable Chemistry and Engineering (2021): ” Enantio-complementary continuous-flow synthesis of 2-aminobutane using covalently immobilized transaminases”
Amazing work with our collaborator Dr. Beatriz Domingues in Johnson Matthey. Chris engineered our evergreen HeWT to make it enantioselective in the synthesis of the smallest chiral amine, 2 amino butane. Well done! You can find the paper here Related Posts
Green Chemistry (2020): “Cell-free biocatalytic synthesis of pipecolic acid: a dual strategy approach and process intensification in flow”
This work was supported by the Wellcome Prime Award Fran received in 2018 through the University of Nottingham. This grant funded Ana for several month as a Post Doc in Nottingham and together with David they worked on a very nice system to produce pipe colic acid (high value molecule) from simple lysine. Liam was a fourth […]
Frontiers Bioeng. Biotechnol. (2020): “An (R)-Selective Transaminase from Thermomyces stellatus: Stabilizing the Tetrameric Form”
Chris expanded our transaminase toolbox by cloning a new (R) selective enzyme which has the advantage of expressing in very high quantities and it’s very stable. Together with out collaborator Louise Gourlay in Milan, we have also successfully crystallised the structure and provided very useful insight into the quaternary structure of such enzyme. The tetrameter could be further stabilised by introducing strategic cysteine residues to enable the formation of intra-subunit […]
Frontiers Bioeng. Biotechnol. (2019): “Enhancing PLP-binding capacity of class-III ω-transaminases by single residue substitution”
The importance of PLP stability in transaminases prompted us to investigate less obvious residues which may be involved in anchoring the PLP. This work was a great collaboration with Dr. Lopez-Gallego (who just recently moved back to San Sebastian) and Dr.Dreveny in Nottingham. Very well done to David who carried out this work and got the good news of it being accepted on the […]
Chemical Science (2019): “Widely applicable background depletion step enables transaminase evolution through solid-phase screening”
This paper describes what appears to be an almost trivial solution to a very well known issue in microbial screening for transaminase activity. Quenching the background with a simple neutralization of the basal pyruvate presence was a stroke of genius! Well done to everyone who contributed! And by the way, we have included also the […]