This paper describes what appears to be an almost trivial solution to a very well known issue in microbial screening for transaminase activity. Quenching the background with a simple neutralization of the basal pyruvate presence was a stroke of genius! Well done to everyone who contributed!
And by the way, we have included also the structure of our transaminase!
Read the paper for free in Chemical Science here
Related posts:
Frontiers Bioeng. Biotechnol. (2019): "Enhancing PLP-binding capacity of class-III ω-transaminases b...
J Flow Chem (2020): "Aromas flow: eco-friendly, continuous, and scalable preparation of flavour este...
Appl Microbiol Biotechnol (2020): "Engineering novel S-glycosidase activity into extremo-adapted β-g...
Advanced Synthesis and Catalysis (2018): Biocatalytic N‐Acylation of Amines in Water Using an Acyltr...
Nature Catalysis (2018): Self-sustaining closed-loop multienzyme-mediated conversion of amines into ...
Trends in Biotechnology (2018): "Flow Bioreactors as Complementary Tools for Biocatalytic Process In...
