Langmuir (2018): Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support
Very nice collaborative work with the group of Ed Magner in Limerick (Ireland). Well done Larah, Cristina and Victoria!
Angew. Chem. Int. Ed. (2018): Carbene in cupredoxin protein scaffolds: replacement of a histidine ligand in the active site substantially alters copper redox properties
Amazing work in collaboration with Martin Albrecht in Bern, Matteo’s incredible dedication to an almost impossible project eventually paid off! Very well done! Check out the paper here
ACS Sustainable Chemistry & Engineering (2018): Self-sufficient flow-biocatalysis by co-immobilisation of pyridoxal 5´-phosphate and ω-transaminases onto porous carriers
Amazing work with our collaborator Fernando Lopez-Gallego in Zaragoza, delighted about this paper which we hope to be the first of many joint publications. Well done to Anabel, Martina and Susana! You can find the paper here
Advanced Synthesis and Catalysis (2018): Biocatalytic N‐Acylation of Amines in Water Using an Acyltransferase from Mycobacterium smegmatis
Well done to Martina, another great paper and a fabulous nice enzyme we think we can incorporate easily in our cascades. You can find the paper here
Scientific Reports (2018): Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form
Very nice to finally have this work out too. Another great collaboration with Prof. Molinari in Milan, here we demonstrate the strategic role of a Phe16 (or equivalent) for the folding of transaminases. We found a native TA which had a threonine at that position and was expressed only insolubly. By replacing the T for […]
ACS Omega (2018): “b-Glucosidase Discovery and Design for the Degradation of Oleuropein”
I am delighted about this work being published. Well done Katie! I was visiting Justin Siegel in UC Davis in the summer of 2015 when a few ideas were put together and from what seemed a very unlikely goal, a lovely paper came out! I am very grateful for all I have learnt while in […]
Chemical Science (2019): “Widely applicable background depletion step enables transaminase evolution through solid-phase screening”
This paper describes what appears to be an almost trivial solution to a very well known issue in microbial screening for transaminase activity. Quenching the background with a simple neutralization of the basal pyruvate presence was a stroke of genius! Well done to everyone who contributed! And by the way, we have included also the […]
Journal of Agricultural and Food Chemistry (2019): “Efficient enzymatic preparation of flavor esters in water”
Lovely collaboration once again with Prof. Francesco Molinari in Milan, well done to everyone involved! Check out the paper at this link
ChemBioChem (2019): “Transaminase-catalyzed continous synthesis of biogenic aldehydes”
Well done to Martina for securing another paper in the field of flow biocatalysis. Here we have looked at the possibility of preparing biogenic aldehydes from biological amines. Exploiting previous work we have co-immobilised the PLP cofactor generating a fully self sufficient reactor. Read the full paper here
Frontiers Bioeng. Biotechnol. (2019): “Enhancing PLP-binding capacity of class-III ω-transaminases by single residue substitution”
The importance of PLP stability in transaminases prompted us to investigate less obvious residues which may be involved in anchoring the PLP. This work was a great collaboration with Dr. Lopez-Gallego (who just recently moved back to San Sebastian) and Dr.Dreveny in Nottingham. Very well done to David who carried out this work and got the good news of it being accepted on the […]