J Flow Chem (2020): “Aromas flow: eco-friendly, continuous, and scalable preparation of flavour esters”
In this manuscript, the core idea of Martina’s Marie Curie project has been captured in its essence with the design of a highly sustainable strategy for the preparation of natural flavour esters, using exclusively reagents found in nature. The precious collaboration with Prof. Molinari and Prof. Tamborini (co-corresponding author) has been key for the optimisation […]
Frontiers Bioeng. Biotechnol. (2019): “Enhancing PLP-binding capacity of class-III ω-transaminases by single residue substitution”
The importance of PLP stability in transaminases prompted us to investigate less obvious residues which may be involved in anchoring the PLP. This work was a great collaboration with Dr. Lopez-Gallego (who just recently moved back to San Sebastian) and Dr.Dreveny in Nottingham. Very well done to David who carried out this work and got the good news of it being accepted on the […]
ChemBioChem (2019): “Transaminase-catalyzed continous synthesis of biogenic aldehydes”
Well done to Martina for securing another paper in the field of flow biocatalysis. Here we have looked at the possibility of preparing biogenic aldehydes from biological amines. Exploiting previous work we have co-immobilised the PLP cofactor generating a fully self sufficient reactor. Read the full paper here
Journal of Agricultural and Food Chemistry (2019): “Efficient enzymatic preparation of flavor esters in water”
Lovely collaboration once again with Prof. Francesco Molinari in Milan, well done to everyone involved! Check out the paper at this link
Chemical Science (2019): “Widely applicable background depletion step enables transaminase evolution through solid-phase screening”
This paper describes what appears to be an almost trivial solution to a very well known issue in microbial screening for transaminase activity. Quenching the background with a simple neutralization of the basal pyruvate presence was a stroke of genius! Well done to everyone who contributed! And by the way, we have included also the […]
Applied Microbiology and Biotechnology (2019): “Haloferax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems”
Very nice to see that Haloferax can indeed catalyse reactions as whole cell organism and be immobilised for reuse too! Great collaboration with our colleague Thorsten Allers here in Nottingham and well done Risat! Link to the paper here Very nice to see that Haloferax can indeed catalyse reactions as whole cell organism and be […]
Catalysts (2019): “Overcoming Water Insolubility in Flow: Enantioselective Hydrolysis of Naproxen Ester”
Very well done David for bringing this paper to a close, the insolubility issue of the substrates was really problematic for flow biocatalysis and the use of small amounts of surfactants solved it much better than high percentage of organic solvents. You can read the paper here
Catalysts (2019): “Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis”
A very nice work where we collaborated with Caroline Paul in Delft to help her assessing the role of biomimetic coenzymes. Full paper here
Frontiers in Microbiology (2019): “Structural characterization of the Xi class glutathione transferase from the haloalkaliphilic archaeon Natrialba magadii”
First one of 2019! This was a nice collaboration where Jenni cloned the gene for Prof. Allocati (University of Chieti-Pescara) so they could do a full characterisation of this novel enzyme. Check it out here
ACS Omega (2018): “b-Glucosidase Discovery and Design for the Degradation of Oleuropein”
I am delighted about this work being published. Well done Katie! I was visiting Justin Siegel in UC Davis in the summer of 2015 when a few ideas were put together and from what seemed a very unlikely goal, a lovely paper came out! I am very grateful for all I have learnt while in […]