Scientific Reports (2018): Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form
Very nice to finally have this work out too. Another great collaboration with Prof. Molinari in Milan, here we demonstrate the strategic role of a Phe16 (or equivalent) for the folding of transaminases. We found a native TA which had a threonine at that position and was expressed only insolubly. By replacing the T for […]
ACS Omega (2018): “b-Glucosidase Discovery and Design for the Degradation of Oleuropein”
I am delighted about this work being published. Well done Katie! I was visiting Justin Siegel in UC Davis in the summer of 2015 when a few ideas were put together and from what seemed a very unlikely goal, a lovely paper came out! I am very grateful for all I have learnt while in […]
OBC (2016) : “Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants”
Well done for Martina e Matteo, the work of the last few months has been successfully accepted by OBC. New paper in our library: Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants. (doi:10.1039/C6OB01629D)
Chemical Science (2019): “Widely applicable background depletion step enables transaminase evolution through solid-phase screening”
This paper describes what appears to be an almost trivial solution to a very well known issue in microbial screening for transaminase activity. Quenching the background with a simple neutralization of the basal pyruvate presence was a stroke of genius! Well done to everyone who contributed! And by the way, we have included also the […]
Green Chemistry (2017) : “Continuous flow biocatalysis: production and in-line purification of amines by immobilised transaminase from Halomonas elongata”
Great achievement for the group! Matteo, Martina and Jenni, together with our collaborators in Milan (Prof. Molinari and Dr. Tamborini) are celebrating a very nice paper on flow chemistry using our transaminase from Halomonas. Well done! The paper “Continuous flow biocatalysis: production and in-line purification of amines by immobilised transaminase from Halomonas elongata” is published […]
Journal of Agricultural and Food Chemistry (2019): “Efficient enzymatic preparation of flavor esters in water”
Lovely collaboration once again with Prof. Francesco Molinari in Milan, well done to everyone involved! Check out the paper at this link
Amino Acids (2017): Stabilization of Angiotensin-(1–7) by key substitution with a cyclic non-natural amino acid
Well done to Anita! Her work finally rewarded! This paper was the result of our collaborative work with Prof. Gallagher at Wake Forest University in North Carolina and the major stabilising effect of incorporating our non natural cyclic amino acid into the Angiotensin sequence, without loosing its anti proliferative properties is a significant step forward […]
ChemBioChem (2019): “Transaminase-catalyzed continous synthesis of biogenic aldehydes”
Well done to Martina for securing another paper in the field of flow biocatalysis. Here we have looked at the possibility of preparing biogenic aldehydes from biological amines. Exploiting previous work we have co-immobilised the PLP cofactor generating a fully self sufficient reactor. Read the full paper here
Chemistry Today (2017): Mini-review “Biocatalysis takes to flow”
F. Paradisi “Biocatalysis takes to flow” Chemistry Today, 2017, 35, 2
Frontiers Bioeng. Biotechnol. (2019): “Enhancing PLP-binding capacity of class-III ω-transaminases by single residue substitution”
The importance of PLP stability in transaminases prompted us to investigate less obvious residues which may be involved in anchoring the PLP. This work was a great collaboration with Dr. Lopez-Gallego (who just recently moved back to San Sebastian) and Dr.Dreveny in Nottingham. Very well done to David who carried out this work and got the good news of it being accepted on the […]